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Purpose(Empty Backbone) Direct cloning and broad-host-range heterologous expression vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAP05
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAP05 was a gift from Bradley Moore (Addgene plasmid # 102920 ; http://n2t.net/addgene:102920 ; RRID:Addgene_102920) -
For your References section:
Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria. Zhang JJ, Tang X, Zhang M, Nguyen D, Moore BS. MBio. 2017 Sep 5;8(5). pii: e01291-17. doi: 10.1128/mBio.01291-17. 10.1128/mBio.01291-17 PubMed 28874475
