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pCAPB02
(Plasmid #65628)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65628 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAPB02
  • Backbone manufacturer
    9800
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    TRP1 ; Spect.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This genes were cloned plasmid pDR111 (gifted from D. Rudner, Harvard Medical School, ref.1) and pCAP01 (ref. 2). Ref.1 Wagner, J. K., Marquis, K. A. & Rudner, D. Z. Sira enforces diploidy by inhibiting the replication initiator dnaa during spore formation in Bacillus subtilis. Mol. Microbiol. 73, 963-974 (2009). Ref. 2 Yamanaka, K., et al. Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc. Natl. Acad. Sci. USA 111, 1957-1962 (2014).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAPB02 was a gift from Pei-yuan Qian (Addgene plasmid # 65628 ; http://n2t.net/addgene:65628 ; RRID:Addgene_65628)
  • For your References section:

    Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis. Li Y, Li Z, Yamanaka K, Xu Y, Zhang W, Vlamakis H, Kolter R, Moore BS, Qian PY. Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383. 10.1038/srep09383 PubMed 25807046