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Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA hEZH2
Plasmid#24230PurposeMammalian expression of human EZH2 with HA tagDepositorInsertEZH2 (EZH2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#183951PurposeInducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorInsertAPOBEC1 (Apobec1 Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)
Viral Prep#100851-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV CAP-B10)
Viral Prep#37825-CAP-B10PurposeReady-to-use AAV CAP-B10 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the CAP-B10 serotype, which permits efficient transduction of both inhibitory and excitatory neurons in the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-T2TP
Plasmid#114725Purposeexpression in mammalian cellsDepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
F2R-Tango
Plasmid#66276PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertF2R (F2R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAJ698
Plasmid#178947PurposeExpresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)DepositorInsertChimeric Tail Fiber Fusion P2-gpH and S16 gp37
UseUnspecifiedTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN
Plasmid#172436PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning siteDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
beta2-spectrin-HA
Plasmid#31070DepositorInsertbeta2-spectrin (SPTBN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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Halo-MTS
Plasmid#124315PurposeExpresses mitochondria specific HaloTag proteinDepositorInsertHalo tag
UseTagsMito specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayTagsExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-c-Myc
Plasmid#13375PurposeRetroviral expression of mouse c-MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer
Plasmid#50920PurposeU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSELIS-RamR
Plasmid#202631PurposeEnables dual growth and fluorescence based enrichment of RamR biosensorsDepositorInsertSh ble
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLambda promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Luc2=tdT
Plasmid#32904PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.DepositorInsertsFirefly Luciferase
tandem Tomato Red Fluorescent Protein
UseTagstdTomato red fluorescent proteinExpressionMammalianMutationPromoterCMV-IE and n/aAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only