168,321 results

1. peGFP C3-SPG20

   Plasmid

   #218578

   Purpose

   Expresses GFP-tagged human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flamindo2

   Plasmid

   #73938

   Purpose

   Expresses biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   Flamindo2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-ER-TurboID-V5_IDG-K

   Plasmid

   #214453

   Purpose

   Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ER-TurboID-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA-gRic6T

   Plasmid

   #106401

   Purpose

   E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

   Depositor

   Sheng Yang

   Article

   Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   pj23119

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p006kanGFP

   Plasmid

   #58534

   Purpose

   GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Green fluorescent protein
   

   Expression

   Bacterial

   Promoter

   T5 between two lac operators

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AICSDP-55:DMD-mEGFP

   Plasmid

   #159744

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_AncBE4max

   Plasmid

   #112094

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_SPP1_WT_V5

   Plasmid

   #82981

   Purpose

   Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SPP1 (SPP1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPaint-mNeon-PuroR

    Plasmid

    #174090

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS mouse IRS-2

    Plasmid

    #11372

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCLX-pTF-R1-DEST-R2-EBR65

    Plasmid

    #45952

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pVITRO1-BFP-CAAX

    Plasmid

    #184459

    Purpose

    Plasma membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-CAAX
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Expression

    Bacterial

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX301-KANK1

    Plasmid

    #121985

    Purpose

    overexpress of KANK1

    Depositor

    GuangJun Zhang

    Article

    Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

    Insert

    KANK1 (KANK1 Human)
    

    Use

    Lentiviral

    Promoter

    CMV promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-iGluSnFR4s-NGR-WPRE (AAV1)

    Viral Prep

    #234437-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-iGluSnFR4s-NGR-WPRE (#234437). In addition to the viral particles, you will also receive purified pAAV-syn-iGluSnFR4s-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4s-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

18. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDisplay-FRISZ

    Plasmid

    #176887

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pDisplay

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Tags

    pDisplay backbone elements for export and membran…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge