164,410 results

1. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

   Plasmid

   #158953

   Purpose

   Express human RAD51(T309A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed threonine 309 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ10878

   Plasmid

   #183964

   Purpose

   CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2T PTP-1B

   Plasmid

   #8602

   Depositor

   Ben Neel

   Insert

   PTP-1B (PTPN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQR

   Plasmid

   #48099

   Purpose

   Cumate-inducible expression of mCherry-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-DsRed

   Plasmid

   #11151

   Purpose

   Mammalian expression vector for expression of DsRed

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   DsRed2 from Discosoma sp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG_IFITM3

   Plasmid

   #179957

   Purpose

   Expression vector for human IFITM3.

   Depositor

   Frank Kirchhoff, Konstantin Sparrer

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE

   Plasmid

   #183522

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   rsChRmine-oScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I146M/G174S

   Promoter

   CaMKIIa

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-UBC-HLA-E SCT-T2A-GFP

   Plasmid

   #205461

   Purpose

   lentiviral plasmid for expression of HLA-E SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HLA-E SCT (HLA-E Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-cmyc

    Plasmid

    #16011

    Purpose

    mammalian expression of human cmyc

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    cmyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-C 16E7

    Plasmid

    #37880

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 16 E7 (E7 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. mEGFP-C1

    Plasmid

    #54759

    Purpose

    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pJL1-eBFP2

    Plasmid

    #102640

    Purpose

    In vitro expression of eBFP2 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    eBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pA-HYG OSCAR

    Plasmid

    #29640

    Depositor

    David_L Andrews, Scott Gold

    Article

    Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. FSW-ALDH2-T2A-EGFP

    Plasmid

    #197036

    Purpose

    Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

    Depositor

    Ronald Hart

    Article

    Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

    Insert

    ALDH2 (ALDH2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPLICSFAST-ER-PO-P2A

    Plasmid

    #214894

    Purpose

    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-PO-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pet28a_Human_H3.3

    Plasmid

    #42632

    Depositor

    Joe Landry

    Insert

    Human H3.3 (H3-3A Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. TFORF0233

    Plasmid

    #142567

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MNX1 (MNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pemiRFP670-N1

    Plasmid

    #136556

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge