248,548 results

1. pBABE BRG1

   Plasmid

   #1959

   Depositor

   Robert Kingston

   Article

   Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

   Insert

   BRG1 (SMARCA4 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E1262K R1575Q

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

   Pooled Library

   #159393

   Purpose

   Mouse lentiviral genome-wide CRISPR library

   Depositor

   Jason Moffat

   Article

   Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-1∆HisTag_hNMT_Nef

   Plasmid

   #66078

   Purpose

   Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

   Depositor

   Dieter Willbold

   Article

   Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

   Inserts

   NMT (NMT1 Human)
   Nef
   

   Expression

   Bacterial

   Mutation

   Deletion of first 80 amino acids

   Promoter

   T7-lacO

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-1∆HisTag_hNMT_Nef

   Plasmid

   #66078

   Purpose

   Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

   Depositor

   Dieter Willbold

   Article

   Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

   Inserts

   NMT (NMT1 Human)
   Nef
   

   Expression

   Bacterial

   Mutation

   Deletion of first 80 amino acids

   Promoter

   T7-lacO

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. phage UbiC scAB-GFP

   Plasmid

   #104998

   Purpose

   Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   single chain Antibody against GCN4
   

   Use

   Lentiviral

   Tags

   GB1, HA tag, and sfGFP

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. phage UbiC scAB-GFP

   Plasmid

   #104998

   Purpose

   Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   single chain Antibody against GCN4
   

   Use

   Lentiviral

   Tags

   GB1, HA tag, and sfGFP

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKC-4.06

    Plasmid

    #112084

    Purpose

    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Mouse, Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKC-4.06

    Plasmid

    #112084

    Purpose

    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Human, Photinus pyralis)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKC-4.04

    Plasmid

    #112085

    Purpose

    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin (HBB Photinus pyralis, Mouse, Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKC-4.04

    Plasmid

    #112085

    Purpose

    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin (HBB Mouse, Human, Photinus pyralis)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. plex_307-ccdB-g418

    Plasmid

    #201141

    Purpose

    pLEX_307 plasmid with g418 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3247

    Plasmid

    #144723

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_SpG‐ABE8e(V106W)

    Plasmid

    #187069

    Purpose

    ABE8e(V106W)-SpG

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpG‐ABE8e(V106W)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RFP-ATF4

    Plasmid

    #125237

    Purpose

    ATF4 expression vector

    Depositor

    Rozita Yarmand

    Article

    Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

    Insert

    ATF4 (ATF4 Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. RFP-ATF4

    Plasmid

    #125237

    Purpose

    ATF4 expression vector

    Depositor

    Rozita Yarmand

    Article

    Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

    Insert

    ATF4 (ATF4 Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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