166,819 results

1. pEGFP-parkin WT

   Plasmid

   #45875

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)

   Viral Prep

   #130995-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE (#130995). In addition to the viral particles, you will also receive purified pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. hSyn-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Promoter

   Syn

   Tags

   mScarlet

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-C3-hYAP1

   Plasmid

   #17843

   Depositor

   Marius Sudol

   Article

   Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211463

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-dead
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   TRE

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Red cGull

    Plasmid

    #191853

    Purpose

    Expresses biosensor for cGMP in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

    Insert

    Red cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid

    #188375

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-GFAP-EGFP

    Plasmid

    #50473

    Purpose

    EGFP under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    GFAP

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIEG3-c-Jun

    Plasmid

    #40348

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    c-Jun (JUN Human)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-HA-ER Y537S

    Plasmid

    #49499

    Purpose

    Expresses HA-tagged ER Y537S mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tyrosine 537 to Serine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gi1-CASE

    Plasmid

    #168120

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A121/E122 within GNAI1

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-EGFP-CAAX

    Plasmid

    #208685

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC5-3XHA-EGFP-PEX26

    Plasmid

    #139054

    Purpose

    HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XHA-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    UBC

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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