pCMV-myc-ERK2-L4A-MEK1_fusion
(Plasmid #39197)

• Depositing Lab: Melanie Cobb
• Publication: Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): TG1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ERK2-MEK1 Fusion
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 2400
• Mutation: L4A = L33A, L37A, L40A, L42A
• GenBank ID: M64300 NM_002755
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
• Entrez Gene: Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

rat ERK2fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES