|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17271||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
/ Fusion Protein
- 6xHis (N terminal on insert)
- Cloning method Restriction Enzyme
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG5-His-SUMO1 was a gift from Stephen Goff (Addgene plasmid # 17271 ; http://n2t.net/addgene:17271 ; RRID:Addgene_17271)
For your References section:Interaction of moloney murine leukemia virus capsid with Ubc9 and PIASy mediates SUMO-1 addition required early in infection. Yueh A, Leung J, Bhattacharyya S, Perrone LA, de los Santos K, Pu SY, Goff SP. J Virol. 2006 Jan . 80(1):342-52. 10.1128/JVI.80.1.342-352.2006 PubMed 16352559