168,558 results

1. His6_BcLOV4_mCherry_BamUK

   Plasmid

   #114596

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Tags

   His6 and mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-Tol2-PA2-CMV-AB-mCh

   Plasmid

   #160435

   Purpose

   Expresses human Amyloid Beta-mCherry in Zebrafish

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_dTAG-KANSL3-targeting

   Plasmid

   #170755

   Purpose

   Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   BSD-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   BSD-P2A-2xHA-dTAG

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB451

   Plasmid

   #74833

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CD47-CD4d3+4-bio

   Plasmid

   #73121

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD47 antigen (CD47 Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAS-Ncad-GFP

   Plasmid

   #35986

   Depositor

   James Jontes, Stephen Smith

   Article

   Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

   Insert

   Ncad (cdh2 Zebrafish)
   

   Use

   Gal4 inducible

   Tags

   EGFP

   Promoter

   14xUAS-E1b

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Plasmid

   #175777

   Purpose

   pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA CUP1p-NuiHA kanMX

    Plasmid

    #131169

    Purpose

    PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Tags

    Nui-HA

    Expression

    Yeast

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-MtAcKRS (human-opti)

    Plasmid

    #164195

    Purpose

    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtAcKRS-478(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    D76G; L325M; L329I; Y330F; L333A; C372F

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-SRRM2-mCherry

    Plasmid

    #174089

    Purpose

    Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

    Depositor

    Wesley Sundquist, Katie Ullman

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    SRRM2 (SRRM2 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Sept. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-OVOL2-HA

    Plasmid

    #184737

    Purpose

    Doxycycline-inducible lentiviral expression of OVOL2

    Depositor

    Alessia Ciarrocchi, Gloria Manzotti

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Insert

    ovo like zinc finger 2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-IRES-MRGPRB2-mCherry

    Plasmid

    #213961

    Purpose

    Expresses MRGPRB2 in mammalian cells

    Depositor

    Christa Müller

    Article

    Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

    Insert

    MRGPRB2 (Mrgprb2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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