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Entirely plasmid-based reverse genetics system for rotaviruses.

Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T
Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
89152pCAG-FAST-p10Express p10-FAST in mammalian cells
89160pCAG-D1RExpress vaccinia virus D1R in mammalian cells
89161pCAG-D12LExpress vaccinia virus D12L in mammalian cells
89162pT7-VP1SA11Express Rotavirus SA11 VP1 in mammalian cells
89163pT7-VP2SA11Express Rotavirus SA11 VP2 in mammalian cells
89164pT7-VP3SA11Express Rotavirus SA11 VP3 in mammalian cells
89165pT7-VP4SA11Express Rotavirus SA11 VP4 in mammalian cells
89166pT7-VP6SA11Express Rotavirus SA11 VP6 in mammalian cells
89167pT7-VP7SA11Express Rotavirus SA11 VP7 in mammalian cells
89168pT7-NSP1SA11Express Rotavirus SA11 NSP1 in mammalian cells
89169pT7-NSP2SA11Express Rotavirus SA11 NSP2 in mammalian cells
89170pT7-NSP3SA11Express Rotavirus SA11 NSP3 in mammalian cells
89171pT7-NSP4SA11Express Rotavirus SA11 NSP4 in mammalian cells
89172pT7-NSP5SA11Express Rotavirus SA11 NSP5 in mammalian cells

Antibodies from Article