Addgene: COVID-19-neutralizing antibodies predict disease severity and survival.

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Alejandro Balazs
Garcia-Beltran et al

COVID-19-neutralizing antibodies predict disease severity and survival.
Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB
Cell. 2021 Jan 21;184(2):476-488.e11. doi: 10.1016/j.cell.2020.12.015. Epub 2020 Dec 15. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
164432	pHAGE-CMV-Luc2-IRES-ZsGreen-W	Lentiviral vector backbone encoding firefly luciferase and ZsGreen
164433	pHDM-SARS-CoV-2	Encodes SARS-CoV-2 Spike Protein for pseudovirus production
164434	pHDM-SARS-CoV-2 Δ18	Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164435	pTwist-SARS-CoV-2	Encodes SARS-CoV-2 Spike Protein for pseudovirus production
164436	pTwist-SARS-CoV-2 Δ18	Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164437	pTwist-SARS-CoV-2 Δ18 D614G	Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production
164438	pTwist-WIV1-CoV	Encodes WIV1-CoV Spike Protein for pseudovirus production
164439	pTwist-WIV1-CoV Δ18	Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
164440	pHDM-VSV-G	Encodes VSV-G protein for pseudovirus production
164441	pHDM-Hgpm2	Encodes codon-optimized HIV gagpol for pseudovirus production
164442	pHDM-Tat1b	Encodes HIV-1 Tat accessory protein for pseudovirus production
164443	pRC-CMV-Rev1b	Encodes HIV-1 Rev accessory protein for pseudovirus production

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