scFLARE2
(Plasmid #158700)

• Purpose: Neuronal expression of scFLARE2 component
• Depositing Lab: Alice Ting
• Publication: Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3660
• Total vector size (bp): 7333
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: scFLARE2
• Species: Synthetic
• Insert Size (bp): 3675
• Mutation: Truncated form of TEV protease (220-242 amino acids deleted, low affinity). S219V mutation improves stability, S153N mutation improves catalytic activity. Calmodulin from GCaMP7s
• Promoter: Syn
• Tag / Fusion Protein:
  • HA, Flag, VP16

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Syn-f
• 3′ sequencing primer: PolyA-r

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry