164,410 results

1. pHIS3p:mRuby2-Tub1+3'UTR::URA3

   Plasmid

   #50639

   Depositor

   Wei-Lih Lee

   Article

   Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

   Insert

   mRuby2-Tub1
   

   Use

   Yeast expression

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   HIS3p

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-P-CAG-GFP

   Plasmid

   #80491

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   CAG-GFP
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1885

   Plasmid

   #144114

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZA_CeTRIC-B1_Del

   Plasmid

   #87344

   Purpose

   Inducible expression of CeTRIC-B1 in P.Pichia strains

   Depositor

   Zhenfeng Liu

   Article

   Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

   Insert

   TRIC-B1
   

   Use

   Tags

   polyhistidine

   Expression

   Yeast

   Mutation

   Δ248-297

   Promoter

   AOX1

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS_CMV::rtTA3-bGHpA

   Plasmid

   #62445

   Purpose

   MXS_chaining vector with CMV::rtTA3-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the reverse tetracycline transactivator with CMV promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-FLAG-APEX2-MAP1LC3BK51A

   Plasmid

   #175754

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K51A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-SFB-BRCA2

   Plasmid

   #99395

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

   Depositor

   Junjie Chen, Michael Huen

   Article

   Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_IRF4_WT

   Plasmid

   #81782

   Purpose

   Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-KP1

    Plasmid

    #206504

    Purpose

    AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    KP1 capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAE1 G8.3 gRNA

    Plasmid

    #90777

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NAE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NAE1 (Guide Designation G8.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 IRF9-STAT1C

    Plasmid

    #37543

    Depositor

    Curt Horvath

    Article

    Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

    Insert

    IRF-9 STAT1 C (IRF9, STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmini-iCAP-AAV.CAP-B22

    Plasmid

    #175005

    Purpose

    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti puro EF2 LUC-2A-Dendra2

    Plasmid

    #129427

    Purpose

    expression of LUC and Dendra2 reporters under EF2 promoter

    Depositor

    Oskar Laur

    Insert

    LUC Dendra2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid

    #211796

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ-5S-F30-Mango IV

    Plasmid

    #127584

    Purpose

    Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    5S rRNA with F30 folding scaffold and Mango IV tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-KG-WASP-GBD

    Plasmid

    #30113

    Depositor

    David Sacks

    Article

    Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

    Insert

    WASP-GBD (WAS, WAS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains the Cdc42-interacting domain of WASP (aa…

    Promoter

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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