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pcDNA3-Flag-RPA2
(Plasmid #22893)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22893 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA2
  • Alt name
    RPA34
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    810
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is one point mutation in the insert, but the protein expressed with this construct forms complexes with the other subunits of the RPA heterotrimer, binds other known interacting proteins (XPA, Tipin), and shows an enrichment in the chromatin fraction of cells treated with replication stress-inducing agents. So by these criteria the construct appears to be WT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-RPA2 was a gift from Aziz Sancar (Addgene plasmid # 22893 ; http://n2t.net/addgene:22893 ; RRID:Addgene_22893)
  • For your References section:

    The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK. Mol Cell Biol. 2007 Apr . 27(8):3131-42. 10.1128/MCB.02190-06 PubMed 17296725