166,819 results

1. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. R777-E172 Hs.PIK3R1-nostop

   Plasmid

   #70456

   Purpose

   Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPlacPbuCas13b-gRNA-T-deGFP

   Plasmid

   #184840

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-NSP3SA11

   Plasmid

   #89170

   Purpose

   Express Rotavirus SA11 NSP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-VP4SA11

   Plasmid

   #89165

   Purpose

   Express Rotavirus SA11 VP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG5-BZLF1

    Plasmid

    #72637

    Purpose

    Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BZLF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q163R

    Promoter

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. yeast Tunable Expression Systems Toolkit (yTEST)

    Plasmid Kit

    #1000000266

    Purpose

    Use this yeast Tunable Expression Systems Toolkit (yTEST) for MoClo assembly of small-molecule inducible promoter systems in S. cerevisiae.

    Depositor

    Jeff Hasty

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

17. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

    Plasmid

    #240799

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen-mCherry-CAAX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

    Plasmid

    #240800

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen mCherry-CAAX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-VDAC1 [N152B/23R-2b]

    Plasmid

    #222171

    Purpose

    Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody. (VDAC1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SGTA-EGFP

    Plasmid

    #210373

    Purpose

    Expresses SGTA fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SGTA (SGTA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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