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pcDNA 3.1(-) VSFP3.1
(Plasmid #18951)


Item Catalog # Description Quantity Price (USD)
Plasmid 18951 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5358
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Voltage-Sensitive Fluorescent Protein 3.1
  • Species
    Ciona intestinalis
  • Insert Size (bp)
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As reported in the accompanying publication (Lundby et al., PLoS ONE, 2008 Jun 25;3(6)), the R217Q mutation has been intently introduced in the S4 domain of Ci-VSP to shift the activation curve of the sensor into the physiological range of neuronal membrane potential.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1(-) VSFP3.1 was a gift from Thomas Knopfel (Addgene plasmid # 18951 ; ; RRID:Addgene_18951)
  • For your References section:

    Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements. Lundby A, Mutoh H, Dimitrov D, Akemann W, Knoepfel T. PLoS ONE. 2008 . 3(6):e2514. 10.1371/journal.pone.0002514 PubMed 18575613