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pcDNA 3.1(-) VSFP2.1
(Plasmid #16255)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16255 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 (-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5317
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSFP2.1
  • Alt name
    Voltage-Sensitive Fluorescent Protein 2.1
  • Species
    Ciona intestinalis
  • Insert Size (bp)
    2253

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA coding sequence for Ci-VSP was a gift from Dr. Okamura (NIPS, Okasaki, Japan). The cDNA coding sequence for Cerulean was a gift from Dr. Piston (Vanderbilt University, USA). The cDNA coding sequence for Citrine was a gift from Dr. Griesbeck (MPI Martinsried, Germany).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Voltage sensor domain of Ci-VSP fused to a pair of fluorescent proteins consisting of a C-terminally truncated CFP (Cerulean) and YFP (Citrine)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1(-) VSFP2.1 was a gift from Thomas Knopfel (Addgene plasmid # 16255 ; http://n2t.net/addgene:16255 ; RRID:Addgene_16255)
  • For your References section:

    Engineering and characterization of an enhanced fluorescent protein voltage sensor. Dimitrov D, He Y, Mutoh H, Baker BJ, Cohen L, Akemann W, Knoepfel T. PLoS ONE. 2007 . 2(5):e440. 10.1371/journal.pone.0000440 PubMed 17487283