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RCAS GFP (CT#22)
(Plasmid #13878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13878 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCAS (A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (destroyed during cloning)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS GFP (CT#22) was a gift from Connie Cepko (Addgene plasmid # 13878 ; http://n2t.net/addgene:13878 ; RRID:Addgene_13878)
  • For your References section:

    Production and design of more effective avian replication-incompetent retroviral vectors. Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL. Dev Biol. 1999 Oct 15. 214(2):370-84. 10.1006/dbio.1999.9432 PubMed 10525341