|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15173||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCMV Sport 6 with U6 promoter
- Backbone size (bp) 5000
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.
See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS6/U6 was a gift from Connie Cepko (Addgene plasmid # 15173 ; http://n2t.net/addgene:15173 ; RRID:Addgene_15173)
For your References section:RCAS-RNAi: a loss-of-function method for the developing chick retina. Harpavat S, Cepko CL. BMC Dev Biol. 2006 . 6():2. 10.1186/1471-213X-6-2 PubMed 16426460
Map uploaded by the depositor.