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(Plasmid #15173)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 15173 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pCMV Sport 6 with U6 promoter
  • Backbone size (bp) 5000
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.

See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS6/U6 was a gift from Connie Cepko (Addgene plasmid # 15173 ; ; RRID:Addgene_15173)
  • For your References section:

    RCAS-RNAi: a loss-of-function method for the developing chick retina. Harpavat S, Cepko CL. BMC Dev Biol. 2006 . 6():2. 10.1186/1471-213X-6-2 PubMed 16426460