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pCMV BAF170-Flag
(Plasmid #19142)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19142 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
  • Alt name
    SMARCC2, BAF170, CRACC2, Rsc8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • Entrez Gene
    SMARCC2 (a.k.a. BAF170, CRACC2, CSS8, Rsc8)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Hong-van Le
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

hunam BAF170 cDNA from pFASTBAC-BAF170 Kingston's lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV BAF170-Flag was a gift from Joan Massague (Addgene plasmid # 19142 ; http://n2t.net/addgene:19142 ; RRID:Addgene_19142)
  • For your References section:

    Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the transforming growth factor beta transcriptional program. Xi Q, He W, Zhang XH, Le HV, Massague J. J Biol Chem. 2008 Jan 11. 283(2):1146-55. 10.1074/jbc.M707479200 PubMed 18003620