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pcDNA6-ARID1A
(Plasmid #39311)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39311 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA6-V5/His.b
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5212
  • Total vector size (bp) 11929
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ARID1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6854
  • GenBank ID
    NM_006015.4
  • Entrez Gene
    ARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd, T7-promoter
  • 3′ sequencing primer V5-rev, BGH-Rev
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 17986, and Dr. Weidong Wang (pCI-neo-BAF250 plasmid) Mol Cell Biol 2000;20:8879–88.
  • Terms and Licenses

Depositor Comments

There are two XhoI sites in the construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6-ARID1A was a gift from Ie-Ming Shih (Addgene plasmid # 39311)
  • For your References section:

    ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401