pcDNA6-ARID1A 1759-2285
(Plasmid #39476)

• Depositing Lab: Ie-Ming Shih
• Depositing Organization: Johns Hopkins University and School of Medicine
  Located in United States of America
• Publication: Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.

Backbone

• Vector backbone: pCDNA6-V5/His.b
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5212
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ARID1A
• Species: H. sapiens (human)
• Mutation: expresses ARID1A aa1759-2285
• GenBank ID: NM_006015.4
• Entrez Gene: ARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd, T7-promoter
• 3′ sequencing primer: BGH-rev, V5-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes the C-terminus of ARID1A.

Note that although the nucleotide sequence cloned in this plasmid starts with aa 1746, the cloning strategy was designed so that aa 1746- 1758 will not be translated, as there is no ATG start site. The actual ATG start site is at aa 1759.

DNA (Catalog # 39476-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 39476-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.