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pLenti-puro
(Plasmid #39481)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39481 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti4/TO/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Removed the Gateway elements between the 2 EcoRV sites and replaced the Zeocin resistance gene with a puromycin resistance gene
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter CMV
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • V5 and 6XHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer V5-rev and BGH-Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481)
  • For your References section:

    ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401