|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26124||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepEGFP-N1 (Clontech)
- Backbone size w/o insert (bp) 3957
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameSynaptophysin GCaMP2
SpeciesR. norvegicus (rat)
Insert Size (bp)2292
Entrez GeneSyp (a.k.a. Syp1)
- Cloning method Restriction Enzyme
- 5′ cloning site AfeI (not destroyed)
- 3′ cloning site Ssp1 (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
ORF frame 3 (624-2915): Rat SyGCaMP2.
Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV::ratSyGCaMP2 was a gift from Leon Lagnado (Addgene plasmid # 26124 ; http://n2t.net/addgene:26124 ; RRID:Addgene_26124)
For your References section:A genetically encoded reporter of synaptic activity in vivo. Dreosti E, Odermatt B, Dorostkar MM, Lagnado L. Nat Methods. 2009 Dec . 6(12):883-9. 10.1038/nmeth.1399 PubMed 19898484