166,819 results

1. pcDNA3.0 FLAG TRPV4

   Plasmid

   #45751

   Depositor

   Robert Lefkowitz

   Article

   Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

   Insert

   TRPV4 (Trpv4 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBZS-M.CviQIX (NDel15)

   Plasmid

   #198357

   Purpose

   Express M.CviQIX (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviQIX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Strep-KIFC1*-mCh

   Plasmid

   #120169

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #126216

   Purpose

   Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. E. coli K-12 MG1655 RARE

   Bacterial Strain

   #61440

   Purpose

   This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

   Depositor

   Kristala Prather

   Article

   Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

   Bacterial Resistance

   None

   Available Since

   Feb. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSIH1-puro-control shRNA

   Plasmid

   #26597

   Purpose

   Lentiviral control shRNA vector with puromycin selection.

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB533-44B12m23-sfGFP

   Plasmid

   #186778

   Purpose

   RNAP2 Ser5ph-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   44B12m23-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV SYN flex PSAM4 GlyR IRES EGFP (AAV5)

   Viral Prep

   #119741-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Ef1a-fDIO mCherry (AAV Retrograde)

    Viral Prep

    #114471-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Flp-dependent)

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

11. Lenti-CMV-PEmax-IRES-ZeoR

    Plasmid

    #207353

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor max (PEmax) enzyme
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. N8his-GFPenhancer-GGGGS4-LaG16

    Plasmid

    #140442

    Purpose

    We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

    Depositor

    Motoyuki Hattori

    Article

    Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

    Insert

    GFPenhancer-GGGGS4-LaG16
    

    Use

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. XLone-Puro Cas13d-eGFP U6 BbsI

    Plasmid

    #155184

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Type

    Empty backbone

    Use

    Unspecified; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-EF1a-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #176757-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8m-WPRE (#176757). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8m-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    EF1a

    Tags

    None

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CamKIIa-jGCaMP8s-WPRE (AAV1)

    Viral Prep

    #176752-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Tags

    None

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

17. HSA1_His

    Plasmid

    #202763

    Purpose

    human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-ESR1-Y537N

    Plasmid

    #116373

    Purpose

    Lentiviral expression of ESR1 Y537N

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y537N

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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