Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #24055)


Item Catalog # Description Quantity Price (USD)
Plasmid 24055 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6321
  • Vector type
    Mammalian Expression ; bicistronic
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    may need up to 2 days to grow
  • Copy number


  • Gene/Insert name
    PIK3C3 and PIK3R4
  • Alt name
    hVps34 and hVps15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)
  • Tags / Fusion Proteins
    • Myc (hVps34) (N terminal on insert)
    • V5/his (hVps15) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (hVps34); NheI (hVps15) (not destroyed)
  • 3′ cloning site AvrII (hVps34); NheI (hVps15) (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ludwig Institute for Cancer Research
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his was a gift from Jonathan Backer (Addgene plasmid # 24055 ; ; RRID:Addgene_24055)
  • For your References section:

    hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Yan Y, Flinn RJ, Wu H, Schnur RS, Backer JM. Biochem J. 2009 Feb 1. 417(3):747-55. 10.1042/BJ20081865 PubMed 18957027