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pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his
(Plasmid #24055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24055 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pVitro2
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 6321
  • Vector type
    Mammalian Expression ; bicistronic
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    low hygro
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    may need up to 2 days to grow
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIK3C3 and PIK3R4
  • Alt name
    hVps34 and hVps15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)
  • Tags / Fusion Proteins
    • Myc (hVps34) (N terminal on insert)
    • V5/his (hVps15) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (hVps34); NheI (hVps15) (not destroyed)
  • 3′ cloning site AvrII (hVps34); NheI (hVps15) (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ludwig Institute for Cancer Research
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his was a gift from Jonathan Backer (Addgene plasmid # 24055 ; http://n2t.net/addgene:24055 ; RRID:Addgene_24055)
  • For your References section:

    hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Yan Y, Flinn RJ, Wu H, Schnur RS, Backer JM. Biochem J. 2009 Feb 1. 417(3):747-55. 10.1042/BJ20081865 PubMed 18957027