166,819 results

1. CMV-Tol2 transposase

   Plasmid

   #158774

   Purpose

   Expression of Tol2 transposase

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   Tol2 tranposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Tol2 codon usage frequency well suitable for muri…

   Promoter

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS9-mCherry-Frame +1

   Plasmid

   #66940

   Purpose

   CRISPaint frame selector +1

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

   Viral Prep

   #105530-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   EGFP

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. negMaLionB

   Plasmid

   #113911

   Purpose

   negative control for Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   negMaLionB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV9)

   Viral Prep

   #35509-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. PB-FLuc+GFPd2

    Plasmid

    #127190

    Purpose

    Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

    Depositor

    Jordan Green

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-FOXA1

    Plasmid

    #153109

    Purpose

    Expression of Flag tagged human FOXA1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA1 (FOXA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and U6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Ezrin-N-14

    Plasmid

    #55043

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IP-TMEM192-mKeima

    Plasmid

    #215358

    Purpose

    Probe to evaluate lysophagic activity

    Depositor

    Tamotsu Yoshimori

    Article

    Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

    Insert

    transmembrane protein 192 (TMEM192 Human)
    

    Use

    Retroviral

    Tags

    mKeima

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-TetON-Puro-HIF1a 3809

    Plasmid

    #118705

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. eGFP-KIF5B

    Plasmid

    #172203

    Purpose

    Expresses KIF5B tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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