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Addgene

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161,670 results

Showing: 5361 - 5380 of 161670 results

  1. pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157027
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    JAG2 (JAG2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157027-plasmid-map-sequence-id-303163

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  4. Epac-S-H170

    Plasmid
    #170352
    Purpose
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170352-plasmid-map-sequence-id-345817

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  6. R777-E202 Hs.PRKAG2-nostop

    Plasmid
    #70486
    Purpose
    Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAG2 (PRKAG2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70486-plasmid-map-sequence-id-125935

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  8. pHOHO2_Hsp82viiA

    Plasmid
    #61708
    Purpose
    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.
    Depositor
    Dan Bolon
    Article
    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:
    Insert
    Hsp82viiA (HSP82 Budding Yeast)
    Use
    Yeast integration vector targeting inserts to the…
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    Heteromeric coiled coil inserted after amino acid…
    Promoter
    TEF1
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    61708-plasmid-map-sequence-id-104523

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  11. pSpCas9(BB)-2A-Blast

    Plasmid
    #118055
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118055-plasmid-map-sequence-id-443898

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  14. pAAV-hSyn-mCherry (AAV1)

    Viral Prep
    #114472-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 5361 - 5380 of 161670 results