168,548 results

1. pcDNA3-ADAM17-HA

   Plasmid

   #65105

   Purpose

   Expression of HA tagged ADAM17 (TACE) in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM17 (ADAM17 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE NFKB-TA-LUC-UBC-dTomato-W

   Plasmid

   #49335

   Purpose

   pHAGE NFKB-TA-LUC-UBC-dTomato-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   NFkB consensus binding sequence
   TA
   Luciferase
   UBC
   dTomato
   

   Use

   Lentiviral

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MGAT-SEP-mRuby3

   Plasmid

   #200937

   Purpose

   cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   MGAT2 (MGAT2 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-BioID2-MCS

   Plasmid

   #74223

   Purpose

   To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Myc and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW002-Pc-mNeonGreen

   Plasmid

   #205016

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p23-NLS-RfxCas13d-mcherry-NLS-Flag

   Plasmid

   #165077

   Purpose

   overexpression of RfxCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   RfxCas13d
   

   Expression

   Mammalian

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCm C-term MBP NikJC199U

   Plasmid

   #174365

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-NLS-GFP (AAV PHP.eB)

    Viral Prep

    #104061-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    CAG

    Tags

    GFP

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pCMV5-Smad7-HA

    Plasmid

    #11733

    Depositor

    Jeff Wrana

    Article

    Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

    Insert

    SMAD7 (SMAD7 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCBL101.1

    Plasmid

    #199724

    Purpose

    A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    Multiple Cloning Site, NPTII
    

    Expression

    Plant

    Mutation

    "GAGACC" to "GATACC"

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0079 RESCUE-S

    Plasmid

    #130662

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-smURFP-IRES-HO-1

    Plasmid

    #80345

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Expression

    Mammalian

    Promoter

    CMV and IRES

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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