PurposeExpresses evoAPOBEC1-BE4max-NG in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125616||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
MutationE4K H109N H122L D124N R154H A165S P201S F205S
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
Please see https://benchling.com/s/seq-YMX6CoDtiqAsxyhvf3mx for additional information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBT375 was a gift from David Liu (Addgene plasmid # 125616 ; http://n2t.net/addgene:125616 ; RRID:Addgene_125616)
For your References section:Continuous evolution of base editors with expanded target compatibility and improved activity. Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. 10.1038/s41587-019-0193-0 PubMed 31332326