Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Cas9-NAT Citations (11)

Plasmid Article: Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
PubMed Journal

Articles Citing Cas9-NAT

Enhanced isoprenoid production from xylose by engineered Saccharomyces cerevisiae. Kwak S, Kim SR, Xu H, Zhang GC, Lane S, Kim H, Jin YS. Biotechnol Bioeng. 2017 Jul 1. doi: 10.1002/bit.26369. PubMed
Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes. Barbieri EM, Muir P, Akhuetie-Oni BO, Yellman CM, Isaacs FJ. Cell. 2017 Nov 30;171(6):1453-1467.e13. doi: 10.1016/j.cell.2017.10.034. Epub 2017 Nov 16. PubMed
Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae. Wei LJ, Kwak S, Liu JJ, Lane S, Hua Q, Kweon DH, Jin YS. Biotechnol Bioeng. 2018 Jul;115(7):1793-1800. doi: 10.1002/bit.26595. Epub 2018 Apr 6. PubMed
Enhanced acetic acid stress tolerance and ethanol production in Saccharomyces cerevisiae by modulating expression of the de novo purine biosynthesis genes. Zhang MM, Xiong L, Tang YJ, Mehmood MA, Zhao ZK, Bai FW, Zhao XQ. Biotechnol Biofuels. 2019 May 10;12:116. doi: 10.1186/s13068-019-1456-1. eCollection 2019. PubMed
Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction. Sun L, Kwak S, Jin YS. ACS Synth Biol. 2019 Sep 20;8(9):2131-2140. doi: 10.1021/acssynbio.9b00217. Epub 2019 Aug 20. PubMed
Identification and characterization of novel xylose isomerases from a Bos taurus fecal metagenome. Tang R, Ye P, Alper HS, Liu Z, Zhao X, Bai F. Appl Microbiol Biotechnol. 2019 Dec;103(23-24):9465-9477. doi: 10.1007/s00253-019-10161-1. Epub 2019 Nov 7. PubMed
Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast. Sun L, Lee JW, Yook S, Lane S, Sun Z, Kim SR, Jin YS. Nat Commun. 2021 Aug 17;12(1):4975. doi: 10.1038/s41467-021-25241-y. PubMed
Chemical rescue of mutant proteins in living Saccharomyces cerevisiae cells by naturally occurring small molecules. Hassell DS, Steingesser MG, Denney AS, Johnson CR, McMurray MA. G3 (Bethesda). 2021 Sep 6;11(9). pii: 6323229. doi: 10.1093/g3journal/jkab252. PubMed
CRISPRi-Guided Metabolic Flux Engineering for Enhanced Protopanaxadiol Production in Saccharomyces cerevisiae. Lim SH, Baek JI, Jeon BM, Seo JW, Kim MS, Byun JY, Park SH, Kim SJ, Lee JY, Lee JH, Kim SC. Int J Mol Sci. 2021 Oct 31;22(21). pii: ijms222111836. doi: 10.3390/ijms222111836. PubMed
Selective production of retinol by engineered Saccharomyces cerevisiae through the expression of retinol dehydrogenase. Lee YG, Kim C, Sun L, Lee TH, Jin YS. Biotechnol Bioeng. 2022 Feb;119(2):399-410. doi: 10.1002/bit.28004. Epub 2021 Dec 11. PubMed
Dissection and enhancement of prebiotic properties of yeast cell wall oligosaccharides through metabolic engineering. Kwak S, Robinson SJ, Lee JW, Lim H, Wallace CL, Jin YS. Biomaterials. 2022 Mar;282:121379. doi: 10.1016/j.biomaterials.2022.121379. Epub 2022 Jan 18. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.