Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64329 | Cas9-NAT | Cas9 expression cassette carried by a yeast single-copy episome plasmid |
| 64330 | gRNA-ura-HYB | ura3 deletion gRNA cassette carried by pRS42H |
| 64331 | gRNA-trp-HYB | trp1 deletion gRNA cassette carried by pRS42H |
| 64332 | gRNA-leu-HYB | leu2 deletion gRNA cassette carried by pRS42H |
| 64333 | gRNA-his-HYB | his3 deletion gRNA cassette carried by pRS42H |