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Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
64329Cas9-NATCas9 expression cassette carried by a yeast single-copy episome plasmid
64330gRNA-ura-HYBura3 deletion gRNA cassette carried by pRS42H
64331gRNA-trp-HYBtrp1 deletion gRNA cassette carried by pRS42H
64332gRNA-leu-HYBleu2 deletion gRNA cassette carried by pRS42H
64333gRNA-his-HYBhis3 deletion gRNA cassette carried by pRS42H

Antibodies from Article