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In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.
Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS
Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
89752pRGEN-CMV-CjCas9Expression of CjCas9 in mammalian cells
89753pU6-Cj-sgRNAU6 promoter driven expression of Cj-SgRNA cloned with BsmbI
89754pET-CjCas9Expression of CjCas9 with His tag in E.coli
137929pAAV-EFS-CjCas9-eGFP-HIF1a Expression of cjCas9 with Hif1a targeting sgRNA

Antibodies from Article