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pET-CjCas9
(Plasmid #89754)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89754 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Genescript
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 8365
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Transform into BL21(DE3) for protein expression and purification purposes
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CjCas9
  • Species
    Campylobacter jejuni
  • Insert Size (bp)
    3048
  • Promoter T7
  • Tags / Fusion Proteins
    • 6XHis (N terminal on backbone)
    • HA (N terminal on backbone)
    • SV40 NLS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (destroyed during cloning)
  • 5′ sequencing primer taatacgactcactatagg
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-CjCas9 was a gift from Seokjoong Kim (Addgene plasmid # 89754 ; http://n2t.net/addgene:89754 ; RRID:Addgene_89754)
  • For your References section:

    In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS. Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500. 10.1038/ncomms14500 PubMed 28220790