-
PurposeExpression of CjCas9 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepcDNA3.1
-
Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6231
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCas9
-
Alt nameCjCas9
-
Alt nameCjeCas9
-
SpeciesCampylobacter jejuni
-
Insert Size (bp)3009
-
Tag
/ Fusion Protein
- SV40-HA (C terminal on insert)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRGEN-CMV-CjCas9 was a gift from Seokjoong Kim (Addgene plasmid # 89752 ; http://n2t.net/addgene:89752 ; RRID:Addgene_89752) -
For your References section:
In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Kim E, Koo T, Park SW, Kim D, Kim K, Cho HY, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim JS. Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500. 10.1038/ncomms14500 PubMed 28220790
