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Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole.
Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT
Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
121178pMK344 (Hygro-P2A-mAID)N-terminal tagging
121179pMK345 (Hygro-P2A-mAID-mClover)N-terminal tagging
121180pMK346 (Hygro-P2A-mAID-mCherry)N-terminal tagging
121181pMK347 (BSD-P2A-mAID)N-terminal tagging
121182pMK348 (BSD-P2A-mAID-mClover)N-terminal tagging
121183pMK349 (BSD-P2A-mAID-mCherry)N-terminal tagging
121184pMK364 (CMV-OsTIR1-loxP-PURO-loxP)OsTIR1 introduction
121185pMK365 (Tet-OsTIR1-loxP-PURO-loxP)OsTIR1 introduction
121186pMK375 (Hygro-P2A-Stag-3FLAG)N-terminal tagging
121187pMK376 (Hygro-P2A-mClover)N-terminal tagging
121188pMK377 (Hygro-P2A-mCherry2)N-terminal tagging
121189pMK388 (mClover-BSD)C-terminal tagging
121190pMK389 (mCherry-BSD)C-terminal tagging
121191pMK390(Stag-3FLAG-BSD)C-terminal tagging
121192pMK391 (mAID-BSD)C-terminal tagging
121193pMK392 (mAID-mClover-BSD)C-terminal tagging
121194pMK393 (mAID-mCherry-BSD)C-terminal tagging
121195pMK394 (mAID-Nluc-Neo)C-terminal tagging
121196pMK401 (Hygro-P2A-mAID-Nluc)N-terminal tagging
121197pMK407 (BSD-P2A-mAID-Nluc)N-terminal tagging
121198pMK408 (mAID-Nluc Hygro)C-terminal tagging
121199pMK409 (mAID-Nluc BSD)C-terminal tagging

Antibodies from Article