|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121185||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth Strain(s)NEB Stable
Copy numberHigh Copy
MutationOsTIR1 codon optimized for human cells
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
This plasmid is a variant of Addgene plasmid 72835.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMK365 (Tet-OsTIR1-loxP-PURO-loxP) was a gift from Masato Kanemaki (Addgene plasmid # 121185 ; http://n2t.net/addgene:121185 ; RRID:Addgene_121185)
For your References section:Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT. Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010. 10.1016/j.ymeth.2019.04.010 PubMed 31026591