PurposeExpression of LOV2 anchored to mitochondria
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||81009||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5040
- Total vector size (bp) 6000
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)432
/ Fusion Protein
- NTOM20 (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer aagtatcgggccctttgtgc
- 3′ sequencing primer GGCAGCCTGCACCTGAGGTTAATCAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTriEx-NTOM20-LOV2 was a gift from Klaus Hahn (Addgene plasmid # 81009 ; http://n2t.net/addgene:81009 ; RRID:Addgene_81009)
For your References section:LOVTRAP: an optogenetic system for photoinduced protein dissociation. Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H, Kuhlman B, Liu R, Danuser G, Hahn KM. Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926. 10.1038/nmeth.3926 PubMed 27427858