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Yong-Su Jin Lab Plasmids


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Title Authors Publication
Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
64329Cas9-NATHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassetteYeast Expression, CRISPRConstruction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. Cas9 expression cassette carried by a yeast single-copy episome plasmid pRS414-TEF1p-Cas9-CYC1t Add to Cart
64330gRNA-ura-HYBgBlock product of ura3 deletion gRNA cassette (Synthetic)Yeast ExpressionConstruction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. ura3 deletion gRNA cassette carried by pRS42H pRS42H Add to Cart
64331gRNA-trp-HYBgBlock product of trp1 deletion gRNA cassette (Synthetic)Yeast ExpressionConstruction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. trp1 deletion gRNA cassette carried by pRS42H pRS42H Add to Cart
64332gRNA-leu-HYBgBlock product of leu2 deletion gRNA cassette (Synthetic)Yeast ExpressionConstruction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. leu2 deletion gRNA cassette carried by pRS42H pRS42H Add to Cart
64333gRNA-his-HYBgBlock product of his3 deletion gRNA cassette (Synthetic)Yeast ExpressionConstruction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. his3 deletion gRNA cassette carried by pRS42H pRS42H Add to Cart