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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
(Plasmid #118154)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118154 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiCRISPRv2
  • Backbone manufacturer
    Feng Zhang (Addgene #52961)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KRAB-dCas9-P2A-BlastR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4907
  • Mutation
    See depositor comments below
  • Promoter EF1a core and U6
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

We have also generated a version of this backbone with the hPGK promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest.

Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR was a gift from Jorge Ferrer (Addgene plasmid # 118154 ; http://n2t.net/addgene:118154 ; RRID:Addgene_118154)