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Jorge Ferrer Lab: Ferrer Lab Plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
118154Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastRCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
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118156Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFPCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
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118157Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
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118158Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
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118159Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
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118160Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
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