168,579 results

1. PT7lacO-EF-Ts-His6

   Plasmid

   #224371

   Purpose

   Express EF-Ts protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-Ts (tsf E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PT7lacO-EF-G-His8

   Plasmid

   #224369

   Purpose

   Express EF-G protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-G (fusA E. coli)
   

   Tags

   His8

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PT7lacO-His6-IF2

   Plasmid

   #224367

   Purpose

   Express IF2 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF2 (infB E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PT7lacO-RF3-His6

   Plasmid

   #224373

   Purpose

   Express RF3 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RF3 (prfB E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PT7lacO-His6-PheRSa-PheRSb

   Plasmid

   #224364

   Purpose

   Express PheRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   PheRS (pheS E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PT7lacO-His6-AsnRS

   Plasmid

   #224363

   Purpose

   Express AsnRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AsnRS (asnS E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PT7lacO-RRF-His6

   Plasmid

   #224374

   Purpose

   Express RRF protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RRF (frr E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PT7lacO-His6-IF3

   Plasmid

   #224368

   Purpose

   Express IF3 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF3 (infC E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PT7lacO-His6-IF1

   Plasmid

   #224366

   Purpose

   Express IF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF1 (infA E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PT7lacO-His6-ThrRS

    Plasmid

    #224365

    Purpose

    Express ThrRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    ThrRS (thrS E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PT7lacO-His6-CKM

    Plasmid

    #224375

    Purpose

    Express CKM protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    CKM (LOC107051134 Chicken)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PT7lacO-His6-AlaRS

    Plasmid

    #224362

    Purpose

    Express AlaRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    AlaRS (alaS E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PT7lacO-His6-NDK

    Plasmid

    #224376

    Purpose

    Express NDK protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    NDK (ndk E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid

    #140984

    Purpose

    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha15-RLuc8 (GNA15 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SepRS(2)/pSertRNA(B4)/EF-Sep

    Plasmid

    #173897

    Purpose

    The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

    Depositor

    Jason W Chin

    Article

    Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

    Inserts

    SepRS(2)
    pSertRNA
    EF-Sep
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-dCas9

    Plasmid

    #112233

    Purpose

    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

    Depositor

    Thomas Gilmore

    Article

    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

    Inserts

    dCas9
    Porumycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed: Aspartic acid 10 to Glycine, Histidine 8…

    Promoter

    EFS-NS

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)

    Plasmid

    #214843

    Purpose

    Expression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(L132C, H134R, T159C)-EYFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-Tet-On shYAP1-3

    Plasmid

    #193666

    Purpose

    Tet inducible knockdown of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH2685

    Plasmid

    #191372

    Purpose

    Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Tetanus Toxin Light Chain
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Punc-4

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcU6_3 MS2 sgRNA

    Plasmid

    #92394

    Purpose

    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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