pBS mShh (CT#258)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13999||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBS SK
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2600
Entrez GeneShh (a.k.a. 9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M100081, ShhNC)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Full length (~250bp 5’ UTR, 1314bp coding, plus ~1kbp 3’ UTR), from Andy McMahon.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS mShh (CT#258) was a gift from Cliff Tabin (Addgene plasmid # 13999 ; http://n2t.net/addgene:13999 ; RRID:Addgene_13999)