pRDA_550
(Plasmid
#203398)
-
PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12a
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepRDA_550
- Total vector size (bp) 12301
-
Vector typeLentiviral, CRISPR
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEnhanced Acidaminococcus Cas12a
-
gRNA/shRNA sequenceBsmBI Cloning cassette
- Promoter EF1a
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.01.03.522655v1 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRDA_550 was a gift from John Doench (Addgene plasmid # 203398 ; http://n2t.net/addgene:203398 ; RRID:Addgene_203398) -
For your References section:
Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform. Anvar NE, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T. bioRxiv. 2023 Sep 5:2023.01.03.522655. doi: 10.1101/2023.01.03.522655. Preprint. 10.1101/2023.01.03.522655 PubMed 36712129
