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(Plasmid #203398)


Item Catalog # Description Quantity Price (USD)
Plasmid 203398 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 12301
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    Enhanced Acidaminococcus Cas12a
  • gRNA/shRNA sequence
    BsmBI Cloning cassette
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDA_550 was a gift from John Doench (Addgene plasmid # 203398 ; ; RRID:Addgene_203398)
  • For your References section:

    Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform. Anvar NE, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T. bioRxiv 2023.01.03.522655 10.1101/2023.01.03.522655