pRDA_550
(Plasmid #203398)

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a
Depositing Lab

John Doench
Publication

Anvar et al bioRxiv 2023.01.03.522655 ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	203398	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pRDA_550
Total vector size (bp) 12301
Vector type
Lentiviral, CRISPR
Selectable markers
Puromycin

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
NEB Stable
Copy number
Unknown

Gene/Insert

Gene/Insert name
Enhanced Acidaminococcus Cas12a
gRNA/shRNA sequence
BsmBI Cloning cassette
Promoter EF1a

Cloning Information

Cloning method Unknown
5′ sequencing primer n/a
3′ sequencing primer n/a

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- pRDA_550.gbk

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.01.03.522655v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pRDA_550 was a gift from John Doench (Addgene plasmid # 203398 ; http://n2t.net/addgene:203398 ; RRID:Addgene_203398)
For your References section:

Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform. Anvar NE, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T. bioRxiv 2023.01.03.522655 10.1101/2023.01.03.522655

pRDA_550 (Plasmid #203398)