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pRDA_052
(Plasmid #136474)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136474 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Puromycin resistance
  • gRNA/shRNA sequence
    BsmBI Cloning cassette
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDA_052 was a gift from John Doench (Addgene plasmid # 136474 ; http://n2t.net/addgene:136474 ; RRID:Addgene_136474)
  • For your References section:

    Optimization of AsCas12a for combinatorial genetic screens in human cells. DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG. Nat Biotechnol. 2020 Jul 13. pii: 10.1038/s41587-020-0600-6. doi: 10.1038/s41587-020-0600-6. 10.1038/s41587-020-0600-6 PubMed 32661438