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Optimization of AsCas12a for combinatorial genetic screens in human cells.
DeWeirdt PC, Sanson KR, Sangree AK, Hegde M, Hanna RE, Feeley MN, Griffith AL, Teng T, Borys SM, Strand C, Joung JK, Kleinstiver BP, Pan X, Huang A, Doench JG
Nat Biotechnol. 2020 Jul 13. pii: 10.1038/s41587-020-0600-6. doi: 10.1038/s41587-020-0600-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
136474pRDA_052Lentiviral expression of AsCas12a gRNA
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136475pRDA_112Lentiviral expression of AsCas12a
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136476pRDA_174Lentiviral expression of EnAsCas12a
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136477pRosetta_v2Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin
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172650Humagne Set CSet C contains best-ranked guides 1 and 4.
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172651Humagne Set DSet D contains best-ranked guides 2 and 3.
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Recombinant Antibodies from Article