Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform.
Anvar NE, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T
bioRxiv. 2023 Sep 5:2023.01.03.522655. doi: 10.1101/2023.01.03.522655. Preprint. PubMed Article
Anvar NE, Lin C, Ma X, Wilson LL, Steger R, Sangree AK, Colic M, Wang SH, Doench JG, Hart T
bioRxiv. 2023 Sep 5:2023.01.03.522655. doi: 10.1101/2023.01.03.522655. Preprint. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203398 | pRDA_550 | All-in-one lentiviral expression of gRNA and EnAsCas12a |
| 209551 | Inzolia library in pRDA_052 | The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs. |
| 209552 | Inzolia library in pRDA_550 | The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs. |