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pcDNA3-HA-ADH
(Plasmid #11610)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11610 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aldehyde dehydrogenase
  • Alt name
    ALDH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1505
  • Mutation
    K412I
  • GenBank ID
    NM_000689
  • Entrez Gene
    ALDH1A1 (a.k.a. ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-ADH was a gift from Steven Johnson (Addgene plasmid # 11610 ; http://n2t.net/addgene:11610 ; RRID:Addgene_11610)