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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 18949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5433
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHypoxia inducible factor 1 alpha
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Alt nameHIF1a
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SpeciesH. sapiens (human)
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Insert Size (bp)2505
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Mutationwt cDNA
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GenBank IDNM_001530
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Entrez GeneHIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
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Tag
/ Fusion Protein
- HA-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site EcoR I (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-HIF1alpha-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18949 ; http://n2t.net/addgene:18949 ; RRID:Addgene_18949) -
For your References section:
Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin WG. Cancer Cell. 2002 Apr . 1(3):237-46. 10.1016/S1535-6108(02)00043-0 PubMed 12086860