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ODD-Luciferase-pcDNA3
(Plasmid #18965)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18965 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5352
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ODD-Firefly Luciferase
  • Alt name
    HIF1a-ODD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2047
  • Mutation
    HIF1a ODD sequence fused in frame to luciferase ORF
  • GenBank ID
    AAA89082
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ODD-Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18965 ; http://n2t.net/addgene:18965 ; RRID:Addgene_18965)
  • For your References section:

    Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. Safran M, Kim WY, O'Connell F, Flippin L, Gunzler V, Horner JW, Depinho RA, Kaelin WG. Proc Natl Acad Sci U S A. 2006 Jan 3. 103(1):105-10. 10.1073/pnas.0509459103 PubMed 16373502