PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26731||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHypoxia Responsive Elements (3)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
Three hypoxia response elements (24-mers) from the Pgk-1 gene upstream of firefly luciferase.
Note that one of the elements is a 22/24 match for the above sequence. This difference is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HRE-luciferase was a gift from Navdeep Chandel (Addgene plasmid # 26731 ; http://n2t.net/addgene:26731 ; RRID:Addgene_26731)
For your References section:PTEN regulates p300-dependent hypoxia-inducible factor 1 transcriptional activity through Forkhead transcription factor 3a (FOXO3a). Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS. Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7. 10.1073/pnas.0706790105 PubMed 18268343