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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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180,496 results

Showing: 5401 - 5420 of 180496 results

  1. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86613-plasmid-map-sequence-id-168851

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  5. N174-MCS (Puro)

    Plasmid
    #81068
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1α
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    81068-plasmid-map-sequence-id-155844

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  6. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Expression
    Mammalian
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74707-plasmid-map-sequence-id-152737

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  11. pCAG-VSVM

    Plasmid
    #64086
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene
    Depositor
    Ian Wickersham
    Insert
    VSV matrix protein
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64086-plasmid-map-sequence-id-107218

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  16. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55127-plasmid-map-sequence-id-311825

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  18. pEGFP-N1 human cofilin S3E

    Plasmid
    #50861
    Purpose
    expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 S3E (CFL1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S3E psuedo-phosporylated, non-activatable
    Promoter
    CMV
    Available since
    Feb. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50861-plasmid-map-sequence-id-133102

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Showing: 5401 - 5420 of 180496 results