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pcDNA3 RRM2
(Plasmid #13796)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13796 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RRM2
  • Alt name
    Ribonucleotide reductase M2 subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1170
  • GenBank ID
    BC001886
  • Entrez Gene
    RRM2 (a.k.a. R2, RR2, RR2M)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Protein Tech Group, Inc.
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 RRM2 was a gift from Edward Whang (Addgene plasmid # 13796 ; http://n2t.net/addgene:13796 ; RRID:Addgene_13796)
  • For your References section:

    RRM2 induces NF-kappaB-dependent MMP-9 activation and enhances cellular invasiveness. Duxbury MS, Whang EE. Biochem Biophys Res Commun. 2007 Mar 2. 354(1):190-196. 10.1016/j.bbrc.2006.12.177 PubMed 17222798